Submitted Manuscripts

Name, last name:	Philip Voronov
ID:	39
Academic degree, academic title, academic interests:	pharmacy, biotechnology
E-mail:

Name, last name:	Natalia Sidorova
ID:	37
Academic degree, academic title, academic interests:	PhD, Associate Professor course Microbiology, Medical Institute, Petrozavodsk State University biotechnology, microbiology
E-mail:

Results of mutagenic effects study of microbial polysaccharides The article presents the results of a study of mutagenic effects of Pseudomonas alcaligenеs polysaccharides. Pseudomonas genus – non-fermentative ubiquitous bacteria, having specific metabolic cycles and unique physical, chemical and biological properties was used as a producer of natural exopolysaccharides. In an experiment using the Ames test, three variants of test compounds were studied: 1. a compound of the Pseudomonas alcaligenes biofilm, 2. exopolysaccharide matrix and the microorganism cell wall compound, and 3. actually the microbial exopolysaccharide. In all cases lack of mutagen action of polysaccharides of Pseudomonas alcaligenes is proved that does them perspective for use as nanomaterials of new generation – alternative wound coverings.
Результаты исследования мутагенного эффекта полисахаридов микробного происхождения В статье приводятся результаты исследования мутагенного эффекта полисахаридов Pseudomonas alcaligens. В качестве природных продуцентов экзополисахаридов использованы представители рода Pseudomonas – неферментирующие убиквитарные бактерии, обладающие специфическими метаболическими циклами и уникальными физико-химическими и биологическими свойствами. В эксперименте с использованием теста Эймса изучено 3 варианта тестируемых соединений: 1. в составе биопленки Pseudomonas alcaligenes, 2. в составе экзополисахаридного матрикса и компоненты клеточной стенки микроорганизма и 3. собственно  микробный экзополисахарид. Во всех случаях доказано отсутствие мутагенного действия полисахаридов Pseudomonas alcaligenes, что делает их перспективными для использования в качестве наноматериалов нового поколения – альтернативных раневых покрытий.

Name, last name:	Younes Ghasemi
ID:	26
Academic degree, academic title, academic interests:	Professor
E-mail:

Name, last name:	Fatemeh Dabbagh
ID:	28
Academic degree, academic title, academic interests:	dr
E-mail:

Name, last name:	Fatemeh Mohammadi
ID:	29
Academic degree, academic title, academic interests:	dr
E-mail:

Name, last name:	Manica Negahdaripour
ID:	31
Academic degree, academic title, academic interests:	dr
E-mail:

Name, last name:	Afshin Borhanihaghighi
ID:	32
Academic degree, academic title, academic interests:	Professor
E-mail:

Name, last name:	Sedigheh Kianpour
ID:	33
Academic degree, academic title, academic interests:	dr
E-mail:

Name, last name:	Mehrzad Banihashemi
ID:	34
Academic degree, academic title, academic interests:	Mr
E-mail:

Neuroprotective effects of Heat shock protein70 The main outcome of stroke is death of brain cells. Heat shock protein 70 (HSP70) is one of the known factors in cell survival that increases in response to the most of neural insults. A variety of cellular and biochemical processes such as chaperone function, anti-apoptotic, anti-necrotic and anti-inflammatory mechanisms occur by HSP70 to protect cells from damage. This review briefly discusses the association between HSP70 and stroke.
Нейропротекторный эффекты белка теплового шока 70 Главным результатом инсульта является гибель клеток головного мозга. Белок теплового шока 70 (HSP70) является одним из известных факторов в выживаемости клеток, что увеличивает в ответ на большую часть нервных инсультов. Разнообразие клеточных и биохимических процессов, таких как функции шаперонов, антиапоптической, анти-некротические и противовоспалительные механизмы происходят с помощью HSP70, чтобы защитить клетки от повреждений. В этом обзоре кратко обсуждается связь между HSP70 и инсульта.

Name, last name:	hashim Alsherees
ID:	25
Academic degree, academic title, academic interests:	M.Sc. of medical microbiologist /Collage of medicine/University of kufa
E-mail:

Molecular study of proteus mirabilis bacteria isolated from urine and wounds in hospitals Al-Najaf province Background: This study aims to find out the relationship between biochemical test and molecular in diagnosis of proteus mirabilis and correlation between them calibrated and the effects on the patients of the difference age groups. Martial and method: The collected of 60 from patients attending (Al-Najaf hospital/Central healthy Laboratories. Samples were wounds swabs and urine. The spec∙imens were directly strea∙ked onto macConkey and blood ag∙ar san∙d and Incu∙bated at 37ºC for 24 h∙ours. Also the isolates were identified dep `ending on morp`hological and biochemical tests as compared with vitak 2. Poly`merase chain reaction (PCR) was us∙ed to de∙tect the ureC and z∙apA genes in the gen`omes of the bacterial str`ains. Results: A total of 60 cases samples were collected from patient attended AL-Najaf Hospital / Central healthy laboratories . Forty five (75%) out of 60 patient by Vitak 2 , and isolated of proteus mirabilis from 42 cases of urine while 18 cases of wound patients. By Vitak test, the highest frequency of proteus in urine [17/19 (89.5% )] was detected among the patients of the 1st age group (10-30 yr.), followed by the patients of the 2nd age group (31-51yr.) [15/19(78.9% )], while the lowest frequency [1/4(25%)] was seen in the patients of the 3rd age group. While showed the highest frequency of positive in wound were the 2nd age group (31-51yr.) [ 6/7(85.7%)] and then the patients of the 1st age group (10-30yr.) [4/4(100%)], while the lowest frequency was seen in the patients of the 3rd age group[2/7(28.6%)]. The percent of urine and wound patients that positively by PCR were 54.5% in urine and 83.3% in wound .This appear the compared of between positive and negative tests with Proteus mirabilis by PCR detected that isolated from urine and wound patients . The detection of Proteus mirabilis genes by PCR technique for positive patients were recorded in ureC gene (33.3 %),zapA (44.5%) and aac(6')-Ib (22.2%) in urine while in wound were recorded ureC gene (50%),zapA ( 30%) and aac(6')-Ib (20%). Conclusion: The correlation between of the Vitak test and biochemical tests with molecular test . Recommendations: To detect genotypes that lead to aminoglocides resistance .
Молекулярное исследование Proteus Mirabilis бактерий, выделенных из мочи и ран в больницах Аль-Наджаф провинции Background: This study aims to find out the relationship between biochemical test and molecular in diagnosis of proteus mirabilis and correlation between them calibrated and the effects on the patients of the difference age groups. Martial and method: The collected of 60 from patients attending (Al-Najaf hospital/Central healthy Laboratories. Samples were wounds swabs and urine. The spec∙imens were directly strea∙ked onto macConkey and blood ag∙ar san∙d and Incu∙bated at 37ºC for 24 h∙ours. Also the isolates were identified dep `ending on morp`hological and biochemical tests as compared with vitak 2. Poly`merase chain reaction (PCR) was us∙ed to de∙tect the ureC and z∙apA genes in the gen`omes of the bacterial str`ains. Results: A total of 60 cases samples were collected from patient attended AL-Najaf Hospital / Central healthy laboratories . Forty five (75%) out of 60 patient by Vitak 2 , and isolated of proteus mirabilis from 42 cases of urine while 18 cases of wound patients. By Vitak test, the highest frequency of proteus in urine [17/19 (89.5% )] was detected among the patients of the 1st age group (10-30 yr.), followed by the patients of the 2nd age group (31-51yr.) [15/19(78.9% )], while the lowest frequency [1/4(25%)] was seen in the patients of the 3rd age group. While showed the highest frequency of positive in wound were the 2nd age group (31-51yr.) [ 6/7(85.7%)] and then the patients of the 1st age group (10-30yr.) [4/4(100%)], while the lowest frequency was seen in the patients of the 3rd age group[2/7(28.6%)]. The percent of urine and wound patients that positively by PCR were 54.5% in urine and 83.3% in wound .This appear the compared of between positive and negative tests with Proteus mirabilis by PCR detected that isolated from urine and wound patients . The detection of Proteus mirabilis genes by PCR technique for positive patients were recorded in ureC gene (33.3 %),zapA (44.5%) and aac(6')-Ib (22.2%) in urine while in wound were recorded ureC gene (50%),zapA ( 30%) and aac(6')-Ib (20%). Conclusion: The correlation between of the Vitak test and biochemical tests with molecular test . Recommendations: To detect genotypes that lead to aminoglocides resistance .

Name, last name:	hashim Alsherees
ID:	25
Academic degree, academic title, academic interests:	M.Sc. of medical microbiologist /Collage of medicine/University of kufa
E-mail:

Molecular study of proteus mirabilis bacteria isolated from urine and wounds in hospitals Al-Najaf province Background: This study aims to find out the relationship between biochemical test and molecular in diagnosis of proteus mirabilis and correlation between them calibrated and the effects on the patients of the difference age groups. Martial and method: The collected of 60 from patients attending (Al-Najaf hospital/Central healthy Laboratories. Samples were wounds swabs and urine. The spec∙imens were directly strea∙ked onto macConkey and blood ag∙ar san∙d and Incu∙bated at 37ºC for 24 h∙ours. Also the isolates were identified dep `ending on morp`hological and biochemical tests as compared with vitak 2. Poly`merase chain reaction (PCR) was us∙ed to de∙tect the ureC and z∙apA genes in the gen`omes of the bacterial str`ains. Results: A total of 60 cases samples were collected from patient attended AL-Najaf Hospital / Central healthy laboratories . Forty five (75%) out of 60 patient by Vitak 2 , and isolated of proteus mirabilis from 42 cases of urine while 18 cases of wound patients. By Vitak test, the highest frequency of proteus in urine [17/19 (89.5% )] was detected among the patients of the 1st age group (10-30 yr.), followed by the patients of the 2nd age group (31-51yr.) [15/19(78.9% )], while the lowest frequency [1/4(25%)] was seen in the patients of the 3rd age group. While showed the highest frequency of positive in wound were the 2nd age group (31-51yr.) [ 6/7(85.7%)] and then the patients of the 1st age group (10-30yr.) [4/4(100%)], while the lowest frequency was seen in the patients of the 3rd age group[2/7(28.6%)]. The percent of urine and wound patients that positively by PCR were 54.5% in urine and 83.3% in wound .This appear the compared of between positive and negative tests with Proteus mirabilis by PCR detected that isolated from urine and wound patients . The detection of Proteus mirabilis genes by PCR technique for positive patients were recorded in ureC gene (33.3 %),zapA (44.5%) and aac(6')-Ib (22.2%) in urine while in wound were recorded ureC gene (50%),zapA ( 30%) and aac(6')-Ib (20%). Conclusion: The correlation between of the Vitak test and biochemical tests with molecular test . Recommendations: To detect genotypes that lead to aminoglocides resistance .
Молекулярное исследование Proteus Mirabilis бактерий, выделенных из мочи и ран в больницах Аль-Наджаф провинции Background: This study aims to find out the relationship between biochemical test and molecular in diagnosis of proteus mirabilis and correlation between them calibrated and the effects on the patients of the difference age groups. Martial and method: The collected of 60 from patients attending (Al-Najaf hospital/Central healthy Laboratories. Samples were wounds swabs and urine. The spec∙imens were directly strea∙ked onto macConkey and blood ag∙ar san∙d and Incu∙bated at 37ºC for 24 h∙ours. Also the isolates were identified dep `ending on morp`hological and biochemical tests as compared with vitak 2. Poly`merase chain reaction (PCR) was us∙ed to de∙tect the ureC and z∙apA genes in the gen`omes of the bacterial str`ains. Results: A total of 60 cases samples were collected from patient attended AL-Najaf Hospital / Central healthy laboratories . Forty five (75%) out of 60 patient by Vitak 2 , and isolated of proteus mirabilis from 42 cases of urine while 18 cases of wound patients. By Vitak test, the highest frequency of proteus in urine [17/19 (89.5% )] was detected among the patients of the 1st age group (10-30 yr.), followed by the patients of the 2nd age group (31-51yr.) [15/19(78.9% )], while the lowest frequency [1/4(25%)] was seen in the patients of the 3rd age group. While showed the highest frequency of positive in wound were the 2nd age group (31-51yr.) [ 6/7(85.7%)] and then the patients of the 1st age group (10-30yr.) [4/4(100%)], while the lowest frequency was seen in the patients of the 3rd age group[2/7(28.6%)]. The percent of urine and wound patients that positively by PCR were 54.5% in urine and 83.3% in wound .This appear the compared of between positive and negative tests with Proteus mirabilis by PCR detected that isolated from urine and wound patients . The detection of Proteus mirabilis genes by PCR technique for positive patients were recorded in ureC gene (33.3 %),zapA (44.5%) and aac(6')-Ib (22.2%) in urine while in wound were recorded ureC gene (50%),zapA ( 30%) and aac(6')-Ib (20%). Conclusion: The correlation between of the Vitak test and biochemical tests with molecular test . Recommendations: To detect genotypes that lead to aminoglocides resistance .

Name, last name:	hashim Alsherees
ID:	25
Academic degree, academic title, academic interests:	M.Sc. of medical microbiologist /Collage of medicine/University of kufa
E-mail:

Molecular study of proteus mirabilis bacteria isolated from urine and wounds in hospitals Al-Najaf province Abstract: Background: This study aims to find out the relationship between biochemical test and molecular in diagnosis of proteus mirabilis and correlation between them calibrated and the effects on the patients of the difference age groups. Martial and method: The collected of 60 from patients attending (Al-Najaf hospital/Central healthy Laboratories. Samples were wounds swabs and urine. The spec∙imens were directly strea∙ked onto mac-Conkey and blood ag∙ar san∙d and Incu∙bated at 37ºC for 24 h∙ours. Also the isolates were identified dep `ending on morp`hological and biochemical tests as compared with vitak 2. Poly`merase chain reaction (PCR) was us∙ed to de∙tect the ureC and z∙apA genes in the gen`omes of the bacterial str`ains. Results: A total of 60 cases samples were collected from patient attended AL-Najaf Hospital / Central healthy laboratories . Forty five (75%) out of 60 patient by Vitak 2 , and isolated of proteus mirabilis from 42 cases of urine while 18 cases of wound patients. By Vitak test, the highest frequency of pro-teus in urine [17/19 (89.5% )] was detected among the patients of the 1st age group (10-30 yr.), fol-lowed by the patients of the 2nd age group (31-51yr.) [15/19(78.9% )], while the lowest frequency [1/4(25%)] was seen in the patients of the 3rd age group. While showed the highest frequency of positive in wound were the 2nd age group (31-51yr.) [ 6/7(85.7%)] and then the patients of the 1st age group (10-30yr.) [4/4(100%)], while the lowest frequency was seen in the patients of the 3rd age group[2/7(28.6%)]. The percent of urine and wound patients that positively by PCR were 54.5% in urine and 83.3% in wound .This appear the compared of between positive and negative tests with Proteus mirabilis by PCR detected that isolated from urine and wound patients . The detection of Proteus mirabilis genes by PCR technique for positive patients were recorded in ureC gene (33.3 %),zapA (44.5%) and aac(6')-Ib (22.2%) in urine while in wound were recorded ureC gene (50%),zapA ( 30%) and aac(6')-Ib (20%). Conclusion: The correlation between of the Vitak test and biochemical tests with molecular test . Recommendations: To detect genotypes that lead to aminoglocides resistance .
Molecular study of proteus mirabilis bacteria isolated from urine and wounds in hospitals Al-Najaf province Abstract: Background: This study aims to find out the relationship between biochemical test and molecular in diagnosis of proteus mirabilis and correlation between them calibrated and the effects on the patients of the difference age groups. Martial and method: The collected of 60 from patients attending (Al-Najaf hospital/Central healthy Laboratories. Samples were wounds swabs and urine. The spec∙imens were directly strea∙ked onto mac-Conkey and blood ag∙ar san∙d and Incu∙bated at 37ºC for 24 h∙ours. Also the isolates were identified dep `ending on morp`hological and biochemical tests as compared with vitak 2. Poly`merase chain reaction (PCR) was us∙ed to de∙tect the ureC and z∙apA genes in the gen`omes of the bacterial str`ains. Results: A total of 60 cases samples were collected from patient attended AL-Najaf Hospital / Central healthy laboratories . Forty five (75%) out of 60 patient by Vitak 2 , and isolated of proteus mirabilis from 42 cases of urine while 18 cases of wound patients. By Vitak test, the highest frequency of pro-teus in urine [17/19 (89.5% )] was detected among the patients of the 1st age group (10-30 yr.), fol-lowed by the patients of the 2nd age group (31-51yr.) [15/19(78.9% )], while the lowest frequency [1/4(25%)] was seen in the patients of the 3rd age group. While showed the highest frequency of positive in wound were the 2nd age group (31-51yr.) [ 6/7(85.7%)] and then the patients of the 1st age group (10-30yr.) [4/4(100%)], while the lowest frequency was seen in the patients of the 3rd age group[2/7(28.6%)]. The percent of urine and wound patients that positively by PCR were 54.5% in urine and 83.3% in wound .This appear the compared of between positive and negative tests with Proteus mirabilis by PCR detected that isolated from urine and wound patients . The detection of Proteus mirabilis genes by PCR technique for positive patients were recorded in ureC gene (33.3 %),zapA (44.5%) and aac(6')-Ib (22.2%) in urine while in wound were recorded ureC gene (50%),zapA ( 30%) and aac(6')-Ib (20%). Conclusion: The correlation between of the Vitak test and biochemical tests with molecular test . Recommendations: To detect genotypes that lead to aminoglocides resistance .

Name, last name:	Hira Mubeen
ID:	20
Academic degree, academic title, academic interests:	MPhil
E-mail:

Characterization, Transformation and Stable Expression Analysis of Sucrose Synthase Gene promoter in Nicotiana Tabacum The present study is focused on stable transformation using GUS expression cassette under selected sucrose synthase gene promoter in Nicotiana tabacum via Agrobacterium mediated transformation. The availability of a broad spectrum of promoters helps to regulate the temporal and spatial expression patterns of transgenes. The Agrobacterium mediated genetic transformation of plants is an important technique used to transfer foreign gene into the host plant genome. The expression of transgenes is regulated by the promoter attached upstream to the gene. Promoters are important in the control of the overall expression profile of a gene. The selected Sucrose Synthase gene promoter was previously cloned in plant expression vector (pGA482). Further, GUS expression cassette was verified through restriction analysis and PCR amplifications. The PCR positive tobacco transgenic plants for Sucrose Synthase expression cassette was obtained and stained to check the GUS expression levels.
Hira The present study is focused on stable transformation using GUS expression cassette under selected sucrose synthase gene promoter in Nicotiana tabacum via Agrobacterium mediated transformation. The availability of a broad spectrum of promoters helps to regulate the temporal and spatial expression patterns of transgenes. The Agrobacterium mediated genetic transformation of plants is an important technique used to transfer foreign gene into the host plant genome. The expression of transgenes is regulated by the promoter attached upstream to the gene. Promoters are important in the control of the overall expression profile of a gene. The selected Sucrose Synthase gene promoter was previously cloned in plant expression vector (pGA482). Further, GUS expression cassette was verified through restriction analysis and PCR amplifications. The PCR positive tobacco transgenic plants for Sucrose Synthase expression cassette was obtained and stained to check the GUS expression levels.

Name, last name:	Firas Rashad Al-Samarai Al-Samarai
ID:	14
Academic degree, academic title, academic interests:	Assist.Prof. PhD, Animal Breeding
E-mail:

EFFECT OF HLADRB1 ON DEVELOPMENT OF ALOPECIA AREATA Alopecia areata (AA) is among the most highly prevalent human organ specific autoimmune diseases, also known spot baldness. The genetic basis of AA is largely unknown and the role of any potential environmental contributors is also unclear. Evidence supporting a genetic basis for AA depends on the heritability in first-degree relatives. A case control comparative study included forty unrelated Iraqi Arab Muslims patients with AA (30 women and 10 men) aged 6-45 years (mean age 35) were included to investigate the genetic burden of HLA*DRB1 in the development of AA formation in Iraqi Arab Muslims patients.The control group was comprised of 30 healthy unrelated sex and age matched volunteers. Genomic DNA was extracted. Amplification and Hybridization was performed using a panel of sequence-specific oligonucleotide probes (SSOP) using HLA-DRB1 amplification and hybridization kits (SSO HLA type DRB1 plus and Mastermix for HLA type DRB1 Amp plus kits -Innogenetics-Belgium) using automated method by AutoLipa – 48 Innogenetics-Belgum. Results showed increased frequency of HLA-DRB1*11:01:01 in patients with AA compared with healthy controls (p P< 0.026, odd ratio=3.285, 95% CI: 1.151 – 9.378). The other allele HLADRB1* 16:01:01 was also increased in AA patients and not detected in control group. The other DRB1 allele groups that have been tested (*03,*014) failed to achieve statistical significance. In conclusion: There is a significant association between AA and HLADRB1*11:01 in Iraqi Arab Muslims patients.
ВЛИЯНИЕ НА РАЗВИТИЕ HLADRB1 очаговая алопеция Алопеция (АА) является одним из наиболее распространенных человеческих органов конкретных аутоиммунных заболеваний, также известный место облысения. Генетическая основа АА в значительной степени неизвестны и роль возможных экологических вкладчиков также неясно. Доказательства, подтверждающие генетическую основу для АА зависит от наследуемости в первой степени родства. Контрольная случай сравнительное исследование включены сорок несвязанных иракских арабов-мусульман пациентов с АА (30 женщин и 10 мужчин) в возрасте 6-45 лет (средний возраст 35) были включены в исследование генетического бремени HLA DRB1 * в развитии образования АА в иракском Арабская мусульмане patients.The контрольная группа состояла из 30 здоровых, не связанной пола и возраста соответствует добровольцев. Геномную ДНК экстрагировали. Усиление и гибридизация была выполнена с использованием панели последовательности конкретных олигонуклеотидных зондов (SSOP), используя HLA-DRB1 усиление и гибридизации комплекты (SSO HLA DRB1 типа плюс и Mastermix для типа HLA DRB1 Amp плюс комплекты -Innogenetics-Бельгия), используя автоматизированный метод с AutoLipa - 48 Innogenetics-Belgum. Результаты показали повышенную частоту HLA-DRB1 * 11: 01: 01 у пациентов с АА по сравнению со здоровыми (р <0,026 Р, нечетного отношение = 3.285, 95% CI: 1.151 - 9,378). Другой аллель HLADRB1 * 16:01:01 была также увеличена в больных АА и не обнаруживается в контрольной группе. Другие группы DRB1 аллелей, которые были протестированы (* 03, * 014) не удалось достичь статистической значимости. В заключение: Существует значительная связь между АА и HLADRB1 * 11: 01 в иракских арабов-мусульман пациентов.

Name, last name:	Firas Rashad Al-Samarai Al-Samarai
ID:	14
Academic degree, academic title, academic interests:	Assist.Prof. PhD, Animal Breeding
E-mail:

The Association between Human Leukocyte Antigen-DRB1 and Vitiligo Human leukocyte antigen (HLA) as part of the immune system has a role in the disease process. Genetic factors play an important role in susceptibility to vitiligo. Our aim in this study is to assess the relationship between HLA-DRB1 alleles frequency in Iraqi patients with vitiligo compared with a healthy control group using the PCR-SSOP method. The patient group consisted of forty Iraqi Arab Muslims patients with vitiligo that consulted the dermatological department in Al-Kindy teaching hospital from September 2013 to June 2015 were assessed for HLA genotyping for HLA-DRB1. A control group consisted of thirty healthy volunteers among the staff of AL-Kindy College of medicine that did not have vitiligo or family history of vitiligo. HLA genotyping for HLA-DRB1 was performed for each patient and for the control persons using PCR with sequence-specific-oligonucleotide primers. Results showed an increase in the frequency of HLA genotype DRB1* 07:0101 (P value= 0.026) and DRB1* 11:0101 (p-value = 0.016) in patients with vitiligo compared with healthy controls. In conclusions, our results suggest an association between HLA-DRB1* 07:0101 and DRB1* 11:0101 and susceptibility to vitiligo.
Ассоциация между человеческими лейкоцитарный антиген-DRB1 и витилиго Лейкоцитарный антиген человека (HLA), как часть иммунной системы играет важную роль в процессе болезни. Генетические факторы играют важную роль в восприимчивости к витилиго. Наша цель в этом исследовании для оценки взаимосвязи между HLA-DRB1 аллелей частоты в иракских пациентов с витилиго по сравнению со здоровой контрольной группы с помощью метода ПЦР-SSOP. Группа состояла из больного сорока иракских арабов-мусульман пациентов с витилиго, что консультации дерматологические отдел в Аль-Кинди клинической больнице с сентября 2013 года по июнь 2015 года были оценены на HLA генотипирования для HLA-DRB1. Контрольная группа состояла из тридцати здоровых добровольцев среди сотрудников Аль-Кинди колледжа медицины, которые не имеют витилиго или семейную историю витилиго. HLA-генотипирование по HLA-DRB1 проводили для каждого пациента и для контроля лиц, использующих ПЦР с сиквенс-специфических олигонуклеотидных праймеров,. Результаты показали увеличение частоты HLA генотипа DRB1 * 07: 0101 (Р = 0,026 значение) и DRB1 * 11: 0101 (р-значение = 0,016) у пациентов с витилиго по сравнению с здоровых людей. В выводах, наши результаты указывают на связь между HLA-DRB1 * 07: 0101 и DRB1 * 11: 0101 и восприимчивости к витилиго.

Name, last name:	Firas Rashad Al-Samarai Al-Samarai
ID:	14
Academic degree, academic title, academic interests:	Assist.Prof. PhD, Animal Breeding
E-mail:

Molecular Markers an Introduction and Applications The dramatic development of molecular genetics has laid the groundwork for genomics. It have introduced new generations of molecular markers for use in the genetic improvement of farm animals. These markers provide more accurate genetic information and better understand of the animal genetic resources. Scientists, unfamiliar with the different molecular techniques tend to get lost as each has its own advantages and disadvantages. This review represents a trail to shade alight on the different types of molecular markers by introducing a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. This review could be helpful to better understand the characteristics of different genetic markers and the genetic diversity of animal genetic resources.
Молекулярные маркеры введения и приложений The dramatic development of molecular genetics has laid the groundwork for genomics. It have introduced new generations of molecular markers for use in the genetic improvement of farm animals. These markers provide more accurate genetic information and better understand of the animal genetic resources. Scientists, unfamiliar with the different molecular techniques tend to get lost as each has its own advantages and disadvantages. This review represents a trail to shade alight on the different types of molecular markers by introducing a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. This review could be helpful to better understand the characteristics of different genetic markers and the genetic diversity of animal genetic resources.

Name, last name:	Firas Rashad Al-Samarai Al-Samarai
ID:	14
Academic degree, academic title, academic interests:	Assist.Prof. PhD, Animal Breeding
E-mail:

MOLECULAR CHARACTERIZATION OF SOME PRODUCTIVITY TRIATS IN MESOPOTAMIAN BUFFALOES (Bubalus bubalis) One of these major genes is growth hormone and growth hormone receptor (GH, GHR) genes which are related to production and reproduction traits in livestock. Associations were analysed between the bovine growth hormone (GH) as well as growth hormone receptor (GHR) combined with their genotypes (GH/AluI& GHR/AluI). There is extensive literature on the genetic polymorphism of GH and GHR in cattle, but a perusal of literature has indicated a paucity of information on these two genes in buffalo. This study aimed to evaluate the genetic polymorphism within growth hormone and its receptor genes in Iraqi buffalo using the PCR-RFLP technique. Genomic DNA extracted from 100 healthy buffaloes and amplified using primers that were designed from the cattle GH and GHR gene sequences. All tested buffalo DNA amplified fragments at 428bp for GH 5th exon. The digestion with AluI endonuclease gave three types of genotypes. The Distribution of the three genotypes and allele frequency was calculated according to Hardy-Weinberg equation, were LL=94 (94.09%), LV=6 (5.82%) and VV=0 (0.09%), so that most of the buffaloes was homozygous (LL) due to the presence of the restriction site at position 52^53 (AG^CT) and less heterozygous was for the valine allele as compared with leucine allele homozygous. The amplified fragments of GHR gene obtained from all tested buffalo DNA at 342-bp were digested with AluI endonuclease. The result showed that all tested buffaloes are genotyped as GG for GHR gene, where amplified fragments were digested into two digested fragments at 241and 101-bp due to the presence of AluI restriction site at position (AG^CT).
Молекулярная характеристика НЕКОТОРЫХ ПРОИЗВОДИТЕЛЬНОСТИ TRIATS В месопотамских буйволов (Bubalus bubalis) Один из этих основных генов гормона роста и гормона роста рецепторов (ГР, GHR) гены, которые связаны с производством и воспроизводства черт в животноводстве. Ассоциации были проанализированы между бычий гормон роста (GH), а также гормона роста рецептора (GHR) в сочетании с их генотипов (GH / GHR AluI & / AluI). Существует обширная литература по генетическим полиморфизмом ГР и РГР крупного рогатого скота, но прочтение литературы показал, недостаточной информацией об этих двух генов в Буффало.     Это исследование направлено на оценку генетического полиморфизма в пределах гормона роста и генов рецепторов ее в иракском буйвола с использованием ПЦР-ПДРФ технику. Геномную ДНК экстрагировали из 100 здоровых буйволов и амплифицировали с использованием праймеров, которые были разработаны из крупного рогатого скота GH и GHR генных последовательностей. Все испытания буйвола ДНК усиливается фрагменты на 428bp для GH 5 экзоне. Переваривание с AluI эндонуклеазы дал три типа генотипов. Распределение генотипов и три аллеля частоты рассчитывали в соответствии с уравнением Харди-Вайнберга, были LL = 94 (94.09%), Л.В. = 6 (5,82%) и В. В. = 0 (0.09%), так что большинство из буйволов был гомозиготным (МР) из-за наличия рестрикционного сайта в положении 52 ^ 53 (АГ ^ КТ) и менее гетерозиготных был для валина аллеля по сравнению с гомозиготными лейцина аллель.      Усиленные фрагменты гена GHR, полученные из всех испытанных буйвола ДНК на 342-BP расщепляли AluI эндонуклеазы. Результат показал, что все испытанные буйволы которые генотипирование, как GG для гена GHR, где амплифицированные фрагменты переваривали в двух расщепленных фрагментов в 241and 101 п.н. из-за присутствия в сайте рестрикции AluI в положении (Ag ^ CT). Odin iz etikh osnovnykh genov gormona rosta i gormona rosta retseptorov (GR, GHR) geny, kotoryye svyazany s proizvodstvom i vosproizvodstva chert v zhivotnovodstve. Assotsiatsii byli proanalizirovany mezhdu bychiy gormon rosta (GH), a takzhe gormona rosta retseptora (GHR) v sochetanii s ikh genotipov (GH / GHR AluI & / AluI). Sushchestvuyet obshirnaya literatura po geneticheskim polimorfizmom GR i RGR krupnogo rogatogo skota, no prochteniye literatury pokazal, nedostatochnoy informatsiyey ob etikh dvukh genov v Buffalo.     Eto issledovaniye napravleno na otsenku geneticheskogo polimorfizma v predelakh gormona rosta i genov retseptorov yeye v irakskom buyvola s ispol'zovaniyem PTSR-PDRF tekhniku. Genomnuyu DNK ekstragirovali iz 100 zdorovykh buyvolov i amplifitsirovali s ispol'zovaniyem praymerov, kotoryye byli razrabotany iz krupnogo rogatogo skota GH i GHR gennykh posledovatel'nostey. Vse ispytaniya buyvola DNK usilivayetsya fragmenty na 428bp dlya GH 5 ekzone. Perevarivaniye s AluI endonukleazy dal tri tipa genotipov. Raspredeleniye genotipov i tri allelya chastoty rasschityvali v sootvetstvii s uravneniyem Khardi-Vaynberga, byli LL = 94 (94.09%), L.V. = 6 (5,82%) i V. V. = 0 (0.09%), tak chto bol'shinstvo iz buyvolov byl gomozigotnym (MR) iz-za nalichiya restriktsionnogo sayta v polozhenii 52 ^ 53 (AG ^ KT) i meneye geterozigotnykh byl dlya valina allelya po sravneniyu s gomozigotnymi leytsina allel'.      Usilennyye fragmenty gena GHR, poluchennyye iz vsekh ispytannykh buyvola DNK na 342-BP rasshcheplyali AluI endonukleazy. Rezul'tat pokazal, chto vse ispytannyye buyvoly kotoryye genotipirovaniye, kak GG dlya gena GHR, gde amplifitsirovannyye fragmenty perevarivali v dvukh rasshcheplennykh fragmentov v 241and 101 p.n. iz-za prisutstviya v sayte restriktsii AluI v polozhenii (Ag ^ CT).

Name, last name:	chadli rabah
ID:	12
Academic degree, academic title, academic interests:	doctor
E-mail:

Bactericidal effect of aqueous extracts of the bark of the pomegranate (Punica granatum L.) on bacteria. This research concerns the study of antibacterial properties of different aqueous extracts of the bark of the pomegranate (Punica granatum L.). Three bacterial strains were used in this test: Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella. Very interesting bactericidal properties of aqueous extracts of the bark of the pomegranate were found on bacteria. The inhibition zones have a very large diameter up to 20 mm and the MIC and MBC are low, of the order of 0.78 mg/ml. This work has shown antibacterial activity against three bacteria may contribute to the fight against infectious diseases, and possibly offer the possibility of using pomegranate peel pharmaceutical and food industries.
Бактерицидное действие водных экстрактов коры граната (Punica граната L.) на бактерии. Это исследование касается изучения антибактериальных свойств различных водных экстрактов коры граната (Punica граната L.). Три бактериальные штаммы были использованы в этом тесте: золотистый стафилококк, синегнойная палочка и сальмонелла. Очень интересные бактерицидные свойства водных экстрактов коры граната были найдены на бактериях. Ингибирования зоны имеют очень большой диаметр до 20 мм, а МИК и МБК низкие, порядка 0,78 мг / мл. Эта работа показала, антибактериальной активностью в отношении трех бактерии могут внести свой вклад в борьбу с инфекционными заболеваниями, и, возможно, предложить возможность использования кожура граната фармацевтической и пищевой промышленности.

Name, last name:	chadli rabah
ID:	12
Academic degree, academic title, academic interests:	doctor
E-mail:

Bactericidal effect of aqueous extracts of the bark of the pomegranate (Punica granatum L.) on bacteria. This research concerns the study of antibacterial properties of different aqueous extracts of the bark of the pomegranate (Punica granatum L.). Three bacterial strains were used in this test: Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella. Very interesting bactericidal properties of aqueous extracts of the bark of the pomegranate were found on bacteria. The inhibition zones have a very large diameter up to 20 mm and the MIC and MBC are low, of the order of 0.78 mg/ml. This work has shown antibacterial activity against three bacteria may contribute to the fight against infectious diseases, and possibly offer the possibility of using pomegranate peel pharmaceutical and food industries.
Бактерицидное действие водных экстрактов коры граната (Punica граната L.) на бактерии. Это исследование касается изучения антибактериальных свойств различных водных экстрактов коры граната (Punica граната L.). Три бактериальные штаммы были использованы в этом тесте: золотистый стафилококк, синегнойная палочка и сальмонелла. Очень интересные бактерицидные свойства водных экстрактов коры граната были найдены на бактериях. Ингибирования зоны имеют очень большой диаметр до 20 мм, а МИК и МБК низкие, порядка 0,78 мг / мл. Эта работа показала, антибактериальной активностью в отношении трех бактерии могут внести свой вклад в борьбу с инфекционными заболеваниями, и, возможно, предложить возможность использования кожура граната фармацевтической и пищевой промышленности. Ключевые слова: Punica граната Л., кора, антибактериальная активность, водные экстракты, ингибирование.

Name, last name:	AJAY TOMER TOMER
ID:	11
Academic degree, academic title, academic interests:	Ph.D
E-mail:

Determination of Effect of Substrate Concentration and Dilution of Inoculums on Population Dynamics of Pseudomonas Fluorescens Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×105) and (201.67×105) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.
Determination of Effect of Substrate Concentration and Dilution of Inoculums on Population Dynamics of Pseudomonas Fluorescens Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×105) and (201.67×105) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.

Name, last name:	AJAY TOMER TOMER
ID:	11
Academic degree, academic title, academic interests:	Ph.D
E-mail:

Determination of Effect of Substrate Concentration and Dilution of Inoculums on Population Dynamics of Pseudomonas Fluorescens Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×105) and (201.67×105) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.
Determination of Effect of Substrate Concentration and Dilution of Inoculums on Population Dynamics of Pseudomonas Fluorescens Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×105) and (201.67×105) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.

Name, last name:	bouzid khadidja
ID:	10
Academic degree, academic title, academic interests:	Dr
E-mail:

Extraction, identification and quantitative HPLC analysis of flavonoids from fruit extracts of Arbutus unedo L from Tiaret area (Western Algeria). The aim of the current study was to evaluate the total phenolic, flavonoid content and to investigate the antioxidant capacities of the fruit extracts of Arbutus unedo L. that grows in Tiaret area (Western Algeria). First we have extracted the fruit by some non-polar solvent (chloroform, ethyl acetate, 1-butanol). Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. Extracts content was determined by using a high-performance liquid chromatography (HPLC)-UV method. The total phenolic contents of A.unedo L. varied between 12.75±0.06 to 34.17±1.36 mg gallic acid equivalent/g of dry weight of extract. The total flavonoid varied from 2.18±0.10 to 6.54±1.14 mg catechin equivalent/g. The antioxidant potential of all extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, the IC50 of acetate ethyl was the best by 0,009 mg/ml may due to the phenolic compound, in the second was the chloroform extract by IC50=0,015mg/ml, in the third was butanol extract by IC50= 0,022 mg/ml and in the last was water extract by IC50= 0,048mg/ml. the antioxidant activity of all extracts was better than ascorbic acid. The extract obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds were identified; they are catechin, apiginin, silybin, fisetine and naringin.
Добыча, идентификация и количественный анализ ВЭЖХ флавоноидов из фруктов экстрактов Arbutus unedo L Тиарет области (Западная Алжир). Цель настоящего исследования состояла в том, чтобы оценить общее фенольные, содержание флавоноидов и исследовать антиоксидантными потенциала плодовых экстрактов Arbutus unedo L., которые растут в Тиарет области (Западная Алжир). Сначала были извлечены плод некоторой неполярном растворителе (хлороформ, этилацетат, 1-бутанол). Общее содержание фенольных и общее содержание флавоноидов были оценены в соответствии с процедурой Фолин-Ciocalteu и колориметрическим методом, соответственно. Содержание экстрактах определяли с помощью жидкостной хроматографии высокого разрешения (ЖХВР) -УФ метод. Валовое содержание фенольных из A.unedo L. колебалась от 12,75 ± 0,06 до 34,17 ± 1,36 мг эквивалента галловая кислота / г сухого веса экстракта.Общая флавоноид варьировала от 2,18 ± 0,10 до 6,54 ± 1,14 мг катехинов экв / г. Антиоксидант потенциал всех экстрактов оценивали с использованием 1,1-дифенил-2-пикрилгидразила (ДФПГ) свободных радикалов активность, IC50 из этилацетат был лучшим по 0009 мг / мл, могут из-за фенольного соединения, во втором былоэкстракт хлороформа IC50 = 0,015 мг / мл, в третий экстракта бутанола по IC50 = 0022 мг / мл, а в прошлом был водный экстракт по IC50 = 0,048 мг / мл. антиоксидантная активность экстрактов из всех было лучше, чем аскорбиновой кислоты. Экстракт, полученный при оптимальных условиях анализировали с помощью ВЭЖХ и пять флавоноидные соединения были идентифицированы; они катехин, apiginin, силибин, fisetine и нарингин.