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«European Journal of Molecular Biotechnology» (Европейский журнал молекулярной биотехнологии) – научный журнал.

ISSN 2310-6255. E-ISSN 2409-1332

Периодичность – 2 раза в год.
Издается с 2013 года.

Образец


UDC 57.05: 616.72-008.8

 

Production Technology and Physicochemical Properties

of Composition Containing Surfactant Proteins

 

Valery V. Novochadov a , * , Pavel A. Krylov a

 

Volgograd State University, Russian Federation

 


* Corresponding author

E-mail addresses:   (V.V. Novochadov), (P.A. Krylov)

 

 

Abstract

The article describes a production method of substance containing great amount of phospolipids (up to 36 %) and surfactant proteins (up to 2 %) in terms of lyophilisate composition. Basic physical and chemical characteristics of the substance (density, viscosity, surface tension and the coefficient of sliding friction) indicate a high lubricant capacity of the derived product. These properties are kept when mixed with native human synovial fluid in the ratio of 1 to 9 inclusive. The obtained data allows to consider the derived composition, containing surfactant proteins and phospholipids, a variety of bionic lubricant suitable for testing as a potential equivalent of synovial fluid which can be used in traumatology and orthopedics, a cosmetic component or agent which increases the stability of the cell suspension during culturing in bioreactors.

Keywords: pulmonary surfactant, surfactant proteins, phospholipids, boundary lubrication, viscosity, tribology, articular cartilage; cartilage tissue engineering, surface friction 

 

1. Introduction

We are now actively developing technologies of the creation and production of synthetic lubricants and their modifications to apply in different lines of industry, agriculture, medicine and veterinary medicine. Talking about the necessity to provide the contact of lubricant with biological objects, we find the biomimetic approach most effective, as it involves the production and application of the lubricants, which properties are maximally close to natural (Wang, 2014; Hwang et al., 2015; Park et al., 2016). One of the most promising sources of natural lubricants are surfactant mixtures produced from mammals' lung containing specific surfactant proteins (SP) and phospholipids (Sarker et al., 2011; Casals, Cañadas, 2012; Schenck, Fiegel, 2016).


2. Material and Methods 

Obtaining substance containing surfactant proteins

The experiment involved 16 white male rats of Wistar line 6 months old weighing 180-240 g Protocol of experiments comply with the ethical standards set out in the "Code of Good Practice in Research involving animal experimentation" and Directive 2010/63 / EU of the European Parliament and of the Council of the European Union on the protection of animals used for scientific purposes. 

3. Results 

The composition of SCSP according to the results of the definition included lipids (mostly - phospholipids), fractions of proteins with mass 16-24 kD (including the subunit SP - B, SP - C) and mass32-48 kD (including SP - D and some of SP - A), a small amount of impurity protein fractions and mineral salts. 

 

4. Discussion

Protein-containing bionic lubricants are able to provide a so-called boundary lubrication that means the lubrication, which is effective at high pressures and minimum layer of lubricating substance (McNary et al., 2012; Lu, 2009; Greene et al., 2011). Thus, hyaluronic acid is responsible for hydrodynamic lubrication at moderate pressures and the sufficient layer of the synovial fluid layer between the joint surfaces, and in case of direct contact of cartilage and high pressure of the contact - specific glycoprotein lubricin weighting 227 kDa (Flannery et al., 2009; Novochadov et al., 2014; Ludwig et al., 2015). Similar mechanisms are required in terms of maintaining the elasticity of the alveoli, which is provided by the surfactant lung system having a high grade of homology with lubricin, primarily due to the presence of mucin domain fragment (Blanco, Pérez-Gil, 2007, Fathi-Azarbayjani, Jouyban, 2015).

 

5. Conclusion

The resulting product has composition with basic properties of a lubricating composite. The main components of SCSP are surfactant-associated proteins and phospholipids. Physical and chemical properties correspond to the characteristics of natural lubricant – the synovial fluid of articular cartilage. Therefore, this product can be tested as a potential component of synovial fluid prosthetic and other biomedical products.

 

6. Acknowledgements

The reported study was particularly supported by Foundation for Assistance to Small Innovative Enterprises in Science and Technology.

 

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УДК 57.05: 616.72-008.8

 

Технология получения и физико-химические свойства композиции,

содержащей белки сурфактанта 

 

Валерий Валерьевич Новочадов a * ,Павел Андреевич Крылов а

 

а Волгоградский государственный университет, Российская Федерация

 

* Корреспондирующий автор

Адреса электронной почты:   (В.В. Новочадов),

(П.А. Крылов)

 

Аннотация. В работе описана методика получения из легких млекопитающих композиции с высоким содержанием фосфолипидов (до 36 %) и белков сурфактанта (до 2 %) в пересчете на лиофилизат. Основные физико-химические характеристики композиции (плотность,  вязкость, коэффициент поверхностного натяжения и коэффициент трения скольжения) свидетельствуют о высокой лубрикативной способности полученного продукта. Эти свойства сохраняются при смешивании с нативной синовиальной жидкостью человека в разведении до 1: 9 включительно. Полученные данные позволяют считать полученную композицию, содержащую фосфолипиды и белки сурфактанта, вариантом бионического лубриканта, пригодного для проведения испытаний в качестве потенциального протеза синовиальной жидкости в травматологии и ортопедии, компонента косметических средств или средства, увеличивающую стабильность клеточной суспензии при культивировании в биореакторах. 

Ключевые слова: легочный сурфактант, белки сурфактанта, фосфолипиды, граничная смазка, вязкость, трение скольжения, трибология, суставной хрящ; тканевая инженерия хряща.


 




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